# Reading list

> The Big Sky Peptides reading list — The Big Sky Peptides reading list: DSIP experiments with light, altitude, rabbits, human sleep, blood degradation and engineered fusion peptides.

**Published studies and reviews**

The artificial-night papers take me furthest into the subject. The rabbit comparisons and the human measurements make the argument harder.

## Light and altitude

The [tree-shrew experiment with a simulated space rhythm](https://pubmed.ncbi.nlm.nih.gov/2278004/) and the [phosphorylated-DSIP study at simulated altitude in rats](https://pubmed.ncbi.nlm.nih.gov/30107169/) anchor my interest in experimental surroundings. A [rat seizure study comparing night and day](https://pubmed.ncbi.nlm.nih.gov/8483798/) adds an especially uncomfortable example of time-dependent effects.

## Rabbits and human sleep

The [original rabbit characterisation](https://pubmed.ncbi.nlm.nih.gov/265572/) starts the DSIP history. I favour reading it alongside the [later rabbit structure–function comparison](https://pubmed.ncbi.nlm.nih.gov/11525128/). For the human questions, the [small insomnia study](https://pubmed.ncbi.nlm.nih.gov/7028502/) and the [sleep-onset plasma measurements](https://pubmed.ncbi.nlm.nih.gov/8745061/) describe importantly different kinds of observation.

## Delivery and unresolved questions

The [blood-incubation experiment](https://pubmed.ncbi.nlm.nih.gov/3628078/) gives the delivery discussion a physical starting point. The [engineered fusion-peptide study in mice](https://pubmed.ncbi.nlm.nih.gov/39444618/) shows how far an altered molecule can take the subject. The [review of DSIP's unresolved biology](https://pubmed.ncbi.nlm.nih.gov/16539679/) is a useful irritant when the individual results begin to sound like a complete explanation.

## References

[1] [Effects of simulated space rhythm (L:D = 0.75h:0.75h) on circadian rhythm in tree shrews (Tupaia belangeri chinensis)] https://pubmed.ncbi.nlm.nih.gov/2278004/
[2] Phosphorylated delta sleep inducing peptide restores spatial memory and p-CREB expression by improving sleep architecture at high altitude https://pubmed.ncbi.nlm.nih.gov/30107169/
[3] Characterization of a delta-electroencephalogram (-sleep)-inducing peptide https://pubmed.ncbi.nlm.nih.gov/265572/
[4] [Hypnogenic properties of DSIP peptide analogs: structural-functional relationship] https://pubmed.ncbi.nlm.nih.gov/11525128/
[5] The influence of synthetic DSIP (delta-sleep-inducing-peptide) on disturbed human sleep https://pubmed.ncbi.nlm.nih.gov/7028502/
[6] Human plasma DSIP decreases at the initiation of sleep at different circadian times https://pubmed.ncbi.nlm.nih.gov/8745061/
[7] Plasma concentration of nine hormones and neurotransmitters during usual activities or constant bed rest for 34 H https://pubmed.ncbi.nlm.nih.gov/7895296/
[8] Delta sleep-inducing peptide (DSIP): a still unresolved riddle https://pubmed.ncbi.nlm.nih.gov/16539679/
[9] Degradation and aggregation of delta sleep-inducing peptide (DSIP) and two analogs in plasma and serum https://pubmed.ncbi.nlm.nih.gov/3628078/
[10] Sleep-wave activity of a delta sleep-inducing peptide analog correlates with its penetrance of the blood-brain barrier https://pubmed.ncbi.nlm.nih.gov/3754348/
[11] Pichia pastoris secreted peptides crossing the blood-brain barrier and DSIP fusion peptide efficacy in PCPA-induced insomnia mouse models https://pubmed.ncbi.nlm.nih.gov/39444618/
[12] Neuropeptides and human sleep https://pubmed.ncbi.nlm.nih.gov/9456470/
[13] Circadian effects of beta-endorphin, melatonin, DSIP, and amphetamine on pentylenetetrazol-induced seizures https://pubmed.ncbi.nlm.nih.gov/8483798/
[14] Expression and Purification of Delta Sleep-Inducing Peptide Fused with Protein Transduction Domain and Human Serum Albumin in Pichia pastoris https://pubmed.ncbi.nlm.nih.gov/28462721/
[15] [The hypnogenic effects of delta sleep-inducing peptide (DSIP) analogs: a comparative study in rabbits and rats] https://pubmed.ncbi.nlm.nih.gov/1455954/

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At Big Sky Peptides the published DSIP experiments take me through artificial nights; the essays here offer no medical advice.